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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Inhibition of STAT3- and MAPK-dependent PGE2 synthesis ameliorates phagocytosis of fibrillar β-amyloid peptide (1-42) via EP2 receptor in EMF-stimulated N9 microglial cells

Fig. 2

Improvement in phagocytic ability for EMF-exposed N9 cells with the addition of celecoxib. N9 cells were pretreated with or without celecoxib (1, 5, and 25 μM) for 30 min and then exposed to 2.45-GHz EMF (+) or sham exposed (−) for 20 min. Then, cells were subjected to a 1-h process of phagocytosis of 647-fAβ42 at the indicated time points after EMF exposure. a Enzyme immunoassay of PGE2 production in N9 cells pretreated with or without celecoxib 3 and 12 h after EMF exposure. b Normalized average fluorescence intensity of 647-fAβ42 ingested per group 12 h after EMF exposure estimated using a flow cytometer and a confocal microscope. For a and b, *P < 0.05 vs the sham-exposed control group; # P < 0.05 vs the EMF-exposed group. c Microscopy images of 647-fAβ42 phagocytosis in N9 cells pretreated with or without celecoxib 12 h after EMF exposure. Scale bar = 20 μm

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Journal of Neuroinflammation

ISSN: 1742-2094