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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Inhibition of STAT3- and MAPK-dependent PGE2 synthesis ameliorates phagocytosis of fibrillar β-amyloid peptide (1-42) via EP2 receptor in EMF-stimulated N9 microglial cells

Fig. 4

Inhibition of JAK2, STAT3, and MAPKs ameliorated the phagocytosis of 647-fAβ42 and abrogated the induction of TNF-α and NO in EMF-stimulated N9 cells. N9 cells were pretreated with or without celecoxib (25 μM), AG490 (25 μM), S3I-201 (30 μM), SB203580 (10 μM), PD98059 (30 μM), and SP600125 (5 μM), for 30 min and then exposed to 2.45-GHz EMF or sham exposed for 20 min. Then, cells were subjected to a 1-h process of phagocytosis of 647-fAβ42 12 h after EMF exposure. a Normalized average fluorescence intensity of 647-fAβ42 ingested per group 12 h after EMF exposure estimated using a flow cytometer. Enzyme immunoassay of PGE2 (b) and TNF-α (c) production and Griess determination of nitrite (d) in N9 cells pretreated with or without the mentioned pharmacologic compounds of interest 12 h after EMF exposure. *P < 0.05 vs the sham-exposed control group; # P < 0.05 vs the EMF-exposed group

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