Fig. 4

Brain vascularization of control and Cstb ^−/− mice. a Histochemical detection of brain vessels in the cortex of control and Cstb ^−/− mice at P14 and P30 was performed using DAB, which detects erythrocytes based on their endogenous peroxidase expression. b Vascularization is quantified at P14 and at P30 as relative DAB-positive area in 64 images from each of four control and four Cstb ^−/− brains. Data are presented as mean ± SEM (**p < 0.01, scale bar = 50 μM)