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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Tollip, an early regulator of the acute inflammatory response in the substantia nigra

Fig. 4

iNOS is increased in the midbrain of Tollip-deficient mice 6 h after LPS intra-nigral injection. a Eleven-month-old female mice underwent PBS (1 μL) or LPS (0.1 μg in 1 μL PBS) injection (n = 7 and n = 8 respectively for WT mice and n = 6 and n = 12 respectively for Tollip KO mice). mRNA amounts in midbrain extracts were normalized by β-actin and GAPDH. *P < 0.05 versus WT mice and ### P < 0.001 versus PBS. b Representative iNOS immunostaining in contralateral and ipsilateral side of the LPS (0.1 μg) injection, in WT and Tollip KO 15-month-old male mice. Magnification = ×40. Scale bar = 30 μm. c Quantification of iNOS-positive cells, performed on two fields in the injected area, on three sections per animal (n = 4 mice in WT and 5 mice in KO group). **P < 0.01 versus WT mice and ## P < 0.01 and ### P < 0.001 versus non-injected side. Box and whiskers show median, min., and max. values, 25th and 75th percentiles. Plots represent individual values and means are represented as a “+.” Two-way ANOVA followed by a Tukey’s post hoc test

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