Variable sensitivity to complement-dependent cytotoxicity in murine models of neuromyelitis optica

Table 1 Summary of cell death in monoculture, neuroglial mixed culture and brain slices

Cell death		Monoculture^a	Mixed culture^b	Brain slices^c
Mouse AST	HC	No death	No death	No death
Mouse AST	#53+HC	33.9 ± 1.5%	32.0 ± 1.7%	Damage from 8 h
Mouse N	HC	93.4 ± 4.0%	31.4 ± 4.0%	17.4 ± 0.7%
Mouse N	#53+HC	97.7 ± 2.1%	72.3 ± 11.1%	52.3 ± 2.7%
Mouse OL	HC	70.8 ± 2.8%	56.0 ± 1.4%	26.4 ± 5.7%
Mouse OL	#53+HC	61.3 ± 7.8%	73.2 ± 5.4%	43.7 ± 3.9%
Mouse OPC	HC	42.2 ± 5.6%	No death	No death
Mouse OPC	#53+HC	36.9 ± 5.6%	No death	46.9 ± 16.9%

Abbreviations: AST astrocyte, N neuron, OL mature oligodendrocyte, OPC oligodendrocyte precursor cell, HC human complement, #53+HC rAb #53 plus human complement.
^aPercentage of cell death at 4 h
^bPercentage death at 4 h except neuron (N) death with #53+HC which was maximal at 1.5 h
^cCell death in brain slices at 48 h

ISSN: 1742-2094