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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Regulation of effector function of CNS autoreactive CD4 T cells through inhibitory receptors and IL-7Rα

Fig. 2

Regulation of IL-7Rα expression in myelin-specific CD4 T cells by T-bet and IL-12. ac Splenocytes from naive TCR-WT and TCR-T-bet−/− mice were activated with MBP Ac1-11 for 72 h (a). The activated cells were then rested for 4 days (b) and reactivated with MBP Ac1-11 for 2 days (c). IL-7Rα expression was determined by flow cytometry. One to two mice from each group were analyzed in each independent experiment and flow data are representative of three independent experiments. The percentage of IL-7Rα expressing cells in T-bet−/− group was normalized to that in WT group, and group means were calculated and compared. dg Splenocytes from naive TCR-WT mice (d) or TCR-T-bet−/− mice (e) were activated with MBP Ac1-11 in the absence or presence of IL-12 for 72 h. The activated cells were then rested for 4 days and reactivated with MBP Ac1-11 for 2 days (f TCR-WT mice, g TCR-T-bet −/− mice). PD-1, LAG-3, and IL-7Rα expression was determined by flow cytometry. One to two mice from each group were analyzed in each independent experiment and flow data are representative of three independent experiments. The percentage of PD-1 and LAG-3 expressing cells or IL-7Rα expressing cells in IL-12 treated group was normalized to that in antigen only group, and group means were calculated and compared. Cells were gated on CD4+ CD44+ cells. All error bars denote s.e.m. *P < 0.05

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