Fig. 3

The expression of PD-1 and IL-7Rα in myelin-specific Th17 cells. a–c Splenocytes from naïve TCR-WT mice were activated with MBP Ac1-11 with different combination of cytokines for 3 days. PD-1 and IL-7Rα expression was determined by flow cytometry. The percentage of PD-1 (b) or IL-7Rα (c) expressing cells in MBP Ac1-11 plus IL-6 group or MBP Ac1-11 plus IL-6/TGFβ group was normalized to that in MBP Ac1-11 group. Group means were calculated and compared. Cells were gated on CD4⁺ CD44⁺ cells, and flow data are representative of three independent experiments. d–f Draining LN cells from immunized WT/B6 (d) or T-bet ^−/−/B6 mice (e) were activated with MOG 35-55 or MOG 35-55 plus IL-23 for 3 days. PD-1 and IL-7Rα expression was determined by flow cytometry. IL-17 in supernatant was determined by ELISA (f). One to three mice from each group were analyzed in each independent experiment and flow data are representative of three independent experiments. All error bars denote s.e.m. *P < 0.05