Fig. 6

Cell cycle inhibition reduces protein expression of microglial/macrophages marker Iba-1 induced by hypobaria exposure after TBI. a Representative immunoblots for Iba-1 and the loading control (GAPDH). b Quantification of the expression levels of Iba-1 protein revealed significant increases in the TBI + Veh + HB group in contrast to the TBI-alone group in the ipsilateral cortex at day 1 after TBI. A significant decrease of Iba-1 expression was observed in the CR8 treatment group in comparison to the TBI/Veh/HB group. N = 7 (sham), 9 (TBI), 10 (TBI//Veh/HB), 7 (TBI/CR8/HB). *p < 0.05, TBI vs. sham injury; ^## p < 0.01, TBI/Veh/HB vs. TBI no HB; ^$$ p < 0.01, TBI/CR8/HB vs. TBI/Veh/HB. c Representative immunofluorescent staining for Iba-1 (green) at 30 days post-injury and DAPI (blue). d, e Quantification of Iba-1⁺ cells revealed significant increases in the TBI + Veh + HB group in contrast to the TBI-alone group. A significant decrease of Iba-1⁺ cells was observed in the CR8 treatment group in comparison to the TBI/Veh/HB group. N = 4 (sham), 6 (TBI), 6 (TBI/Veh/HB), 6 (TBI/CR8/HB). *p < 0.05, TBI vs. sham injury; ^$ p < 0.05, ^$$ p < 0.01, TBI/Veh/HB vs. TBI; ^### p < 0.001, TBI/CR8/HB vs. TBI/Veh/HB. Scale bar = 50 μm