Fig. 9

Cell cycle inhibition reduces protein expression of astrocytes marker GFAP induced by hypobaria exposure following TBI. a Representative immunofluorescent staining for GFAP (green) and DAPI (blue). b, c Quantification of GFAP⁺ cells revealed significant increases in the TBI + Veh + HB group in contrast to the TBI-alone tissue. A significant decrease of GFAP⁺ cells was observed in the CR8 treatment group in comparison to the TBI/HB group. N = 4 (sham), 6 (TBI), 6 (TBI/Veh/HB), 6 (TBI/CR8/HB). *p < 0.05, TBI vs. sham injury; ^$ p < 0.05, TBI/Veh/HB vs. TBI; ^### p < 0.001, TBI/CR8/HB vs. TBI/Veh/HB. Scale bar = 50 μm