Fig. 1

Lrp1 knockdown exacerbates the production of pro-inflammatory cytokines. a–d Primary microglia were transiently transfected with non-targeting siRNA (NT) or two independent Lrp1-specific siRNAs for 48 h, then incubated with serum-free medium in the presence or absence of LPS (100 ng/ml) for 4 h (for qRT-PCR) or 24 h (for ELISA). RNA was extracted and the relative mRNA levels of Lrp1 (a), Il-1β (b), Tnf-α (c), and Il-10 (d) were determined by qRT-PCR and shown as bar graph (n = 3). β-Actin was used as an internal control. IL-1β (e) and TNF-α (f) in conditioned media were determined by ELISA (n = 3). Data were plotted as mean ± SEM and normalized to the corresponding control group. *p < 0.05; **p < 0.01; ***p < 0.001 (one-way ANOVA with post hoc Tukey’s t test)