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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: LRP1 modulates the microglial immune response via regulation of JNK and NF-κB signaling pathways

Fig. 2

Both JNK and NF-κB pathways are activated in Lrp1-deficient primary microglia. a Primary microglia were transiently transfected with non-targeting siRNA (NT) or Lrp1-specific siRNAs for 48 h. Cells were stimulated with 100 ng/mL LPS for the indicated time (min). Cell lysates were prepared and analyzed by Western blotting using antibodies specific for LRP1, NF-κB, JNK, p38, ERK, or the phosphorylated forms of these proteins. β-Actin served as a loading control. bf The quantification of Western blots of LRP1 (b), ratios of phospho-JNK/total JNK (c), phospho-NF-κB/total NF-κB (d), phospho-ERK/total ERK (e), and phospho-p38/total p38 (f). The relative signal intensities of each pathway at various time points were normalized to NT-treated microglia in the absence of LPS stimulation (0 min time point) (n = 3). Data were plotted as mean ± SEM *p < 0.05; **p < 0.01; ***p < 0.001 (one-way ANOVA with post hoc Tukey’s t test)

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