Fig. 6From: LRP1 modulates the microglial immune response via regulation of JNK and NF-κB signaling pathwaysNF-κB inhibitor restores LRP1 expression suppressed by LPS. a–b Primary microglia were pretreated with 10 μM SP600125 or Bay 11-7082 for 30 min, followed by stimulation with LPS (100 ng/mL) or vehicle for 24 h. RNA was extracted, and the relative mRNA levels of Lrp1 in microglia treated with SP600125 (a) and BAY11-7082 (b) were determined by qRT-PCR (n = 3). β-Actin was used as an internal control. Data were plotted as mean ± SEM ***p < 0.001; N.S. not significant (two-tailed Student’s t test). c–f The protein levels of LRP1, phospho-NF-κB, phospho-c-Jun, phospho-IκBα, total NF-κB, total c-Jun, and β-actin in cell lysates were examined by Western blot analysis (c) and quantified (d–f) (n = 3). Data represent mean ± SEM. ***p < 0.001; N.S. not significant (one-way ANOVA with post hoc Tukey’s t test)Back to article page