Table 1 Experimental plan summarizing treatments and experiment (exp.) numbers included in the study
From: Pro-epileptogenic effects of viral-like inflammation in both mature and immature brains
Cell culture experiments | In vivo experiments |
|---|---|
Microglia | Rapid kindling protocol |
RT-PCR: PBS vs. PIC, n = 5 (x3 exp.) | P14: saline (n = 5) vs. PIC (n = 6) |
Luminex: PBS vs. PIC, n = 5 (x1 exp.) | P75: saline (n = 6) vs. PIC (n = 7) |
RT-PCR: PIC vs. PIC + Mino, n = 5 (x3 exp.) | P14: mino (n = 7) vs. PIC + Mino (n = 7) |
Luminex: PIC vs. PIC + Mino, n = (x1 exp.) | P75: mino (n = 5) vs. PIC + Mino (n = 5) |
Macrophages | Cytokines dosage by luminex |
RT-PCR: PBS vs. PIC, n = 5 (x3 exp.) | P14: saline i.p. + saline i.h. (n = 5) |
Luminex: PBS vs. PIC, n = 5 (x1 exp.) | Saline i.p. + PIC i.h. (n = 5) |
RT-PCR: PIC vs. PIC + mino, n = 5 (x3 exp.) | Mino i.p. + saline i.h. (n = 5) |
Luminex: PIC vs. PIC + mino, n = 5 (x1 exp.) | Mino i.p. + PIC i.h. (n = 5) |
P75: Saline i.p. + saline i.h. (n = 5) | |
Saline i.p. + PIC i.h. (n = 5) | |
Mino i.p. + saline i.h. (n = 5) | |
Mino i.p. + PIC i.h. (n = 5) |