Fig. 3

JQ1 treatment inhibits retinal microglial activation in the rd10 retina. Intravitreal injection of JQ1 (or vehicle) was performed at PN14, as described in Fig. 1. Eyeballs were collected at PN18, PN24, and PN30. Cryosections were used for immunostaining and fluorescence microscopy. a–c Representative immunostaining images of microglial markers. Scale bar 50 μm. Blue DAPI staining of nuclei. d Quantification: IBA1, CD68, or TSPO positive cells per 500 μm ONL length; mean ± SEM, n = 6 mice. Quantification of IBA1 staining at all five time points (PN18-PN30) is presented in Additional file 1: Figure S3