Fig. 3
ARMS2 binding to cell surfaces. a Binding of ARMS2 (500 nM, 1 h) to naive RPE-cells and (b) human erythrocytes. c ARMS2 binding to untreated (n = 3), early apoptotic (n = 4), late apoptotic (n = 3), and necrotic (n = 4) T cells. T cells were gated according to their staining with annexin V-pacific blue and PI (upper panels), flow cytometry of indicated gates are shown (lower panels). ARMS2 bound in a dose-dependent manner (***p = 0.0001 for necrotic and ***p = 0.0002 for late apoptotic cells, each 10 nM versus 500 nM ARMS2). Background binding of the antibody to the cells is marked (co). d ARMS2 binding for 1 h to CHO-K1 (n = 4), CHOpgsA, or CHOpgsD cells (each n = 5). Flow cytometry experiments show the median MFI values ± s.d. using ARMS2 antiserum (***p = 0.0001, 10 nM versus 500 nM ARMS2). e ARMS2 binding to heparin coated beads (n = 3, median MFI values ± s.d., ***p = 0.0002) and in competition with free heparan sulfate (4–12 mM, n = 3, ***p = 0.0002, 0 mM versus 12 mM heparan sulfate, and **p = 0.016, 4 mM versus 12 mM heparan sulfate). Competition is shown in percent of binding of ARMS2 to the beads alone (100%)