Fig. 2
From: Resolvin RvD2 reduces hypothalamic inflammation and rescues mice from diet-induced obesity
The hypothalamic expression of proteins involved in the synthesis and action of RvD2. The schematic representation of the main enzymes involved in the synthesis and the receptor for RvD2 (a). The transcript expressions of phospholipase A2 (b), 15-lipoxigenase (c), 5-lipoxigenase (d) and GPR18 (e) were evaluated using real-time PCR in samples collected from the hypothalamus of mice fed either chow (CT) or a high-fat diet (HF) by the time specified in the graphics (b–e). To measure hypothalamic RvD2, we employed a MALDI method, with mass spectra standard of RvD2 corresponding to m/z 375 ((f), upper panel); a sample from the hypothalamus of a mice fed on HF ((f), lower panel); quantification of RvD2 in hypothalamic samples (g). In all experiments, n = 7. In b–e and g, *p < 0.05 vs. respective CT