Fig. 5

The impact of exogenous RvD2-injected icv on inflammatory and metabolic parameters in mice. Six-week-old Swiss mice were included in the study and fed a HF diet for 8 weeks before intracerebroventricular (icv) cannulation; after 1 week, mice were randomly selected for either saline (2 μl) or different amounts of RvD2 (2, 3 or 50 ng) icv treatment for 11 days (a). At the end of the experimental period, hypothalamic RNA was extracted and employed in real-time PCR determinations of GPR18 (b). Cumulative food intake (c) and body mass variation (d) were determined during the experimental period. At the end of the experimental period, the epididymal fat pad was measured (e). In addition, mice were submitted a glucose tolerance test, and results are expressed as the area under the curve (AUC) (f). Interleukin-6 (IL6) (g) and interleukin-10 (IL10) (h) transcripts were determined in samples from the hypothalamus, whereas uncoupling protein 1 (UCP1) (i) and PGC1a (j) transcripts were determined in samples from the brown adipose tissue. Some mice were subjected to indirect calorimetry, resulting in the values for O₂ consumption (k), CO₂ production (l) and respiratory quotient (m). In addition, some mice were subjected to a leptin tolerance test (LTT) and results are expressed as cumulative food intake during 12 h (n). In the experiments reported in panels a–j, n = 8; in the experiments reported in panels k–n, n = 5. *p < 0.05 vs. saline. Experiments for evaluation of 3 and 50 ng RvD2 in the hypothalamus were performed in different occasions. The differences obtained in some of the saline groups reflect interexperimental variability. For statistical analysis, group-specific saline control was considered as baseline