Fig. 6

Effects of EP2 antagonist on LPS-induced protein expression in microglia. a LPS increases the expression of NOX2 protein at 24 h. b This effect is reduced by the EP2 antagonist (1 μM PF 04418948). c Also, LPS increases VEGFA protein expression and the EP2 antagonist attenuates this effect (d). e The EP2 antagonist reduces the nitrite production in the microglial culture medium induced by LPS at 48 h, indicating that EP2 contributes to LPS-induced iNOS activity. The figure illustrates a representative experiment (n = 7–11) out of the three independent experiments. a, c n = 3–6 in two to four independent experiments; b, d n = 3 obtained in three independent experiments. *p ≤ 0.05; **p < 0.01. “EP2 antag’ indicates EP2 antagonist