Fig. 7

Effect of APAP on the expression of Bax/Bcl-2 and neuron apoptosis in mice with hippocampal impairment. a–c Protein bands on the gel and their relative intensities on postoperative day 1. d–f The expression levels of Bax/Bcl-2 proteins were normalized to that of β-actin as an internal control. g CA1, CA3 and DG regions of hippocampal from indicated group of mice. TUNEL staining demonstrated neuron apoptosis on postoperative day 1. h The number of hippocampal apoptotic neurons on postoperative day 1 under ×400 magnification. Scale bars: ×100, 200 μm; ×400, 50 μm. Data are expressed as the mean ± SE (n = 6). **P < 0.01, ***P < 0.001 vs. CON group; ^# P < 0.05, ^## P < 0.01, ^### P < 0.001 vs. LPS group. APAP acetaminophen, aCSF artificial cerebrospinal fluid, LPS lipopolysaccharide, SE standard error