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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: WWL70 attenuates PGE2 production derived from 2-arachidonoylglycerol in microglia by ABHD6-independent mechanism

Fig. 5

The highly selective ABHD6 inhibitor KT182 did not reduce PGE2 production. a Activity-based protein profiling with the ABHD6-specific probe HT-01 shows reduced ABHD6 activity in the membrane fraction of BV2 cells in the presence of KT182. b Using membrane fraction from BV2 cells, treatment with KT182, WWL70, or MAFP for 5 min showed decreased hydrolysis of 2-AG by quantifying released radioactive glycerol as described in Fig. 1. Relative 2-AG hydrolysis as compared to the non-treated group are shown (mean ± SD; n = 3). c BV2 cells were incubated with DMEM plus WWL70 (10 μM) or KT182 (100 nM) for 15 min, before addition of 10 μM 2-AG or AA for 15 min, followed by addition of LPS (100 ng/ml) for 18 h. Culture medium was assayed for PGE2 by EIA (mean ± SD, n = 3). Single, double, and triple asterisks denote p < 0.05, 0.01, and 0.001, respectively

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