Fig. 2
Quantification of immune cell accumulation and relative cytokine gene expression in the infarcted hemisphere at day 1 after stroke. a Representative CD11b-immunoreactivity 24 h after tMCAO of mice treated with rat IgG Fab (Ctrl Fab) or p0p/B Fab (GPIb). Scale bar, 100 μm. c Schematic view of the brain regions analyzed to quantify the density of immune cells per mm2. b, d, e Quantification of CD11b-positive cells per slice and CD11b-positive cells/mm2 in different cortical and basal ganglial regions in the ipsilateral hemisphere at day 1 (n = 5/group). f Representative immunocytologic stainings of brain-infiltrating CD3-positive T lymphocytes on day 1 after tMCAO of mice, treated with rat IgG Fab (Ctrl Fab) or p0p/B Fab (GPIb). Scale bar, 25 μm. g–i Quantification of CD3-positive cells per slice and CD3-positive cells/mm2 in different cortical and basal ganglial regions in the ipsilateral hemisphere at day 1 (n = 5/group). j, k Relative gene expression of tumor necrosis factor-α (TNFα), interleukin-1β (IL1β), and interleukin-6 (IL6) in the cortical and basal ganglia ischemic hemispheres of mice, treated with rat IgG Fab (Ctrl Fab) or p0p/B Fab (GPIb) (n = 6/group). *P < 0.05, ***P < 0.001, unpaired Student’s t test