Fig. 4

Knockdown of P2Y6R reduced the production of inflammatory cytokines. a RT-PCR and western blot analyses of P2Y6R from BV-2 cells transfected with P2Y6 siRNA. b Representative western blots showing iNOS and COX-2 levels from LPS-stimulated BV-2 cells transfected with P2Y6 siRNA. c Representative RT-PCR showing the expression levels of inflammatory cytokines (TNF-α, iNOS, IL-6, COX-2, and MIP-2) from LPS-stimulated BV-2 cells transfected with P2Y6R siRNA. The band intensity was quantified using studio lite imager and is presented relative to the level of β-actin. Data are shown as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs Con, ^# p < 0.05, ^## p < 0.01 vs LPS