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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Activation of human B cells negatively regulates TGF-β1 production

Fig. 1

IL-10–producing B cells from human blood. a Frequencies of IL-10-producing B cells from human blood. Cells were cultured with medium or with combined CpG-B + anti-Ig for 24 h. PMA/ionomycin and monensin were added 5 h before the end of the culture. Cells were stained for surface CD19 and CD27 and intracellular IL-10 (top panels) or matched isotype control (bottom panels). The representative dot plots show frequencies of IL-10-producing cells among CD19+CD27 or CD19+CD27+ B cells. b The bar graph indicates mean (± SEM) percentages of B cells that labeled positively for IL-10 (n = 6). Percentages presented are corrected for isotype control staining. c Line plot of frequencies of IL-10-producing cells among CD19+CD27 or CD19+CD27+ B cells. Paired data are shown interconnected. d The bar graph indicates quantification (mean ± SEM) of IL-10 staining in both CD19+CD27 and CD19+CD27+ B cells (n = 6). Geometric mean fluorescence intensities (MFI) were normalized to the isotype control. e The line plot shows normalized IL-10 MFI by cells among CD19+CD27 and CD19+CD27+ B cells. Paired data are shown interconnected. Significant differences using Student’s t test between sample means are indicated

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