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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Activation of human B cells negatively regulates TGF-β1 production

Fig. 2

Combined CpG-B + anti-Ig stimulation potently trigger IL-6 and TNF production by B cells in vitro. Frequencies of (a) IL-6- and (b) TNF-producing B cells from human blood. Cells were cultured with medium or with combined CpG-B + anti-Ig for 24 h. PMA/ionomycin and monensin were added 5 h before the end of the culture. Cells were stained for surface CD19 and CD27 and intracellular IL-6 or TNF (top panels) or matched isotype control (bottom panels). The representative dot plots show frequencies of IL-6- and TNF-producing cells among CD19+CD27 or CD19+CD27+ B cells

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