Fig. 5

Stimulation of B cells restrains LAP expression. a Frequencies of LAP-producing B cells from human blood. Cells were cultured with medium or with combined CpG-B + anti-Ig for 24 h. PMA/ionomycin was added 5 h before the end of the culture. Cells were stained for surface CD19, CD27, and LAP (top panels) or matched isotype control (bottom panels). The representative dot plots show frequencies of LAP–expressing cells among CD19⁺CD27⁻ or CD19⁺CD27⁺ B cells. b The bar graph indicates mean (± SEM) percentages of B cells that labeled positively for LAP (n = 6). Percentages presented are corrected for isotype control staining. c Line plot of frequencies of LAP-expressing cells among CD19⁺CD27⁻ or CD19⁺CD27⁺ B cells. Paired data are shown interconnected. d The bar graph indicates quantification (mean ± SEM) of LAP staining in both CD19⁺CD27⁻ and CD19⁺CD27⁺ B cells (n = 6). Geometric MFI were normalized to the isotype control. e Line plot shows normalized LAP MFI by cells among CD19⁺CD27⁻ and CD19⁺CD27⁺ B cells. Paired data are shown interconnected. Significant differences using Student’s t test between sample means are indicated