Fig. 3
From: Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7
Induction of NFκB target genes by ethanol involves TLR7. HEC slices were exposed to ethanol for 48 h. mRNA was isolated from the tissue as described in the “Methods” section. Additional groups were incubated with siRNA against TLR7 (siTLR7) to assess for involvement of TLR7 role in induction of NFκB genes. a Ethanol caused phosphorylation of the NFκB-p65, evidenced by a 2.2-fold increase in pNFκB (p65) relative to controls (***p < 0.0003, t test). Insert shows representative western blot samples of pNFκB-p65 and β-actin. b MyD88 mRNA was increased by ethanol by 87% of control. siRNA against TLR7 returned MyD88 to near control levels. c TNFα mRNA was induced by ethanol by 246% at 48 h of ethanol exposure; 246.4 ± 10.243% vs 100.7 ± 11.8%, Ethanol vs Control, mean ± SEM. siRNA against TLR7 significantly reduced TNFα mRNA levels to 146% of control. Error bars denote mean ± SEM, ***p < 0.001 vs control, ###p < 0.001 vs ethanol. N = 2–10 slices per group