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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7

Fig. 4

Ethanol causes microvesicle (MV) release of let-7b and HMGB1 from microglia. a MVs were isolated from hippocampal-entorrhinal slice culture (HEC) media after ethanol exposure. let-7b was increased in MVs 3.7-fold by ethanol. b Ethanol increased let-7b in BV2 microglia-derived MVs by 3-fold; 326.7 ± 76.3% vs 100 ± 18.2%, Ethanol vs Control, mean ± SEM, *p < 0.05, t-test. c SH-SY5Y neurons were treated with 100 mM ethanol for 24 h. Ethanol had no effect on let-7b release in MVs from SH-SY5Y neurons; 95.75 ± 5.2% vs 100 ± 8.1%, Control vs Ethanol, mean ± SEM. d Ethanol increased HMGB1 secretion into HEC culture media in a dose dependent fashion. e 72.9% of HMGB1+ MVs from HEC media are microglial-derived, whereas astrocytes and neurons comprised 15.3 and 11.8%, respectively. One-way ANOVA with Sidak’s multiple comparisons test, ****p < 0.00001. f Representative histogram of one ethanol treated sample showing populations of astrocytic (GFAP, dark gray), microglial (CD11b, light blue), and neuron-derived HMGB1+ MVs (Na/K ATPase α3, light gray). *p < 0.05, **p < 0.01, mean ± SEM, N = 3–6 per group

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