Fig. 5

Ethanol alterations in miRNA expression and chaperone protein binding in microvesicles (MVs). Hippocampal-entorhinal (HEC) slices were treated with ethanol (100 mM) and RNA immunopurification (RIP) assay was utilized to assess let-7b complex formation with chaperone proteins. a RIP assay found that ethanol increases the association of let-7b with HMGB1 in MVs by 50%. b Ethanol reduces let-7b binding to argonaut (Ago2) by 50%. c Ethanol caused a robust increase in miR-155 in BV2 microglial MVs. d miR-155 binding to Ago2 was increased in HEC media MVs after ethanol treatment. Mean ± SEM, *p < 0.05, N = 3–6 per group