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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Complex regulation of neutrophil-derived MMP-9 secretion in central nervous system tuberculosis

Fig. 3

MAP-kinase pathways are activated in neutrophils in TB and regulate MMP-9 secretion. a Representative immunoblots of p-38, ERK, and JNK MAP kinases in neutrophils stimulated with CoMTB. CoMTB increases phosphorylation of all pathways from 15 min. Densitometric analysis of response to CoMCont and CoMTB stimulation of b phospho-p38 to total p38, c phospho-ERK to total ERK, and d phospho-JNK to total JNK. e CoMTB-stimulated MMP-9 secretion is suppressed by p-38 inhibitor SB203580. f MMP-9 secretion with CoMTB is inhibited by ERK inhibitor PD98059. g Representative immunoblots to detect p-38, ERK MAP kinases in neutrophils stimulated with M.tb. Densitometric analysis of response to control (PBS) and M.tb infection of h phospho-p-38 to total p-38 and i phospho-JNK to total JNK. j Pre-incubation with p-38 inhibitor SB does not suppress MMP-9 secretion in M.tb-infected neutrophils. k Pre-incubation with ERK inhibitor PD does not affect MMP-9 secretion by M.tb-stimulated neutrophils. All chemical inhibitor experiments were performed by pre-incubating neutrophils for 30 min before stimulating for 4 h. Bars represent mean ± s.d and are representative of at least two independent experiments performed in triplicate. *P < 0.05; **P < 0.01; ***P < 0.001

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