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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Aging and amyloid β oligomers enhance TLR4 expression, LPS-induced Ca2+ responses, and neuron cell death in cultured rat hippocampal neurons

Fig. 6

Aβo increase Ca2+ responses to LPS in aged cultures of rat hippocampal neurons. Hippocampal neurons cultured for >18 DIV were treated for 48 h with vehicle or Aβo (1 μM), loaded with fura2 and subjected to Ca2+ imaging. Pictures show pseudocolor images and representative recordings of [Ca2+]cyt (Ratio F340/F380) before (basal) and after LPS addition (LPS) added as indicated in vehicle (a) and Aβo treated (b) cultured hippocampal neurons. Pseudocolor scales are shown at the right. Bars represent 10 μm. c Bars represent average rises in [Ca2+]cyt calculated as the product of the cell fraction responsive to LPS alone (75%) or LPS + Aβo (85%) by the Δ[Ca2+]cyt recorded in responsive cells (mean ± SEM; n = 62 and 54 cells, respectively, from five independent cultures; *p < 0.05 compared to vehicle)

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