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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Aging and amyloid β oligomers enhance TLR4 expression, LPS-induced Ca2+ responses, and neuron cell death in cultured rat hippocampal neurons

Fig. 7

Effect of in vitro aging on LPS and Aβo induced apoptosis in cultured rat hippocampal neurons. Primary hippocampal neurons cultured for 5–8 (young) and >18 (aged) DIV were treated for 48 h with vehicle (control), LPS (1 μg/ml), Aβo (1 μM) or LPS plus Aβo added together. 48 h later, cell apoptosis was assessed by immunofluorescence of Annexin V. Percent of apoptotic neurons in young (a) and aged (b) cells are shown. Values represent mean ± SEM of three and five experiments from independent cultures, respectively. *p < 0.05 vs. control group; # p < 0.05 vs. LPS group; p < 0.05 vs. Aβo group. c Fold increases in percent of apoptotic cells were calculated from the same data set by dividing the percent of apoptotic cells in each condition by the percent of apoptotic cells observed in young neurons in control conditions. *p < 0.05 vs. LPS group; # p < 0.05 vs. aging alone; p < 0.05 vs. Aβo group in aging

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