Fig. 3

IL-1β attenuates BDNF-induced phosphorylation of retrograde signaling target Erk5. a Levels of phos-Erk5 were measured in cultured neurons with BDNF or BDNF + IL-1β treatments. Neurons treated with BDNF exhibited twofold increase in phos-Erk5 levels over baseline non-treated controls while BDNF + IL-1β treatment exhibited only a 1.3-fold increase in phos-Erk5 levels over baseline, suggesting IL-1β attenuates BDNF-induced phosphorylation of Erk5. Mean ± SE; BDNF = 2.0 ± 0.2, n = 4 independent cultures vs BDNF + IL-1β = 1.1 ± 0.2, n = 3 independent cultures, unpaired two-tailed t test, p < 0.05). b Levels of phos-Erk5 in response to BDNF in the presence of Ciliobrevin D (BDNF + CiB-D)B were reduced to baseline: mean ± SE; BDNF = 1.9 ± 1.4-fold, n = 5 independent cultures vs BDNF + CiB-D = 1.1 ± 0.25-fold (by unpaired t test p < 0.05) c Levels of phos-Erk5 in ex vivo slices with BDNF (left hemisphere) or BDNF + IL-1β (right hemisphere) compared in adult rats that exhibited 14 ± 2% decrease in phos-Erk5 levels after treatment with BDNF + IL-1β when compared to BDNF-only treatment. Graph rescaled such that xBDNF=1.0. Mean ± SE; BDNF = 1.0 ± .02-fold, n = 4 animals vs BDNF + IL-1β = 0.84 ± .02-fold, n = 4 animals, paired t test: p < 0.05)