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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: IL-1β impairs retrograde flow of BDNF signaling by attenuating endosome trafficking

Fig. 6

UCH-L1 rescued the IL-1β effect on BDNF-induced Erk5 phosphorylation. phos-Erk5 activation was measured in somal compartment. a Low magnification images of neurons immunolabeled with antibody against phos-Erk5 or imaged with phase-contrast filter (middle). The images show somal and axonal compartments, which were separated by microgrooves (between the dash lines). Scalebar, 100 μm. b The representative images that demonstrate that BDNF led to an increase in somal phos-Erk5. IL-1β decreased BDNF-induced elevation of somal phos-Erk5 levels. In the presence of UCH-L1, the inhibitory effect of IL-1β was reduced. phos-Erk5 immunoreactivity was normalized to the nuclear counterstain, TO-PRO 3. c Quantification of somal phos-Erk5 levels. BDNF treatment significantly increased somal phos-Erk5 levels (*, p < 0.05, n = 3). In the presence of IL-1β, BDNF treatment did not lead to an elevation of somal phos-Erk5 levels. In the presence of UCH-L1 and IL-1β, BDNF treatment increased somal phos-Erk5 levels (*, p < 0.05, n = 3). Scalebar, 20 μm. Veh, vehicle

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