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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Cytotoxic T cells modulate inflammation and endogenous opioid analgesia in chronic arthritis

Fig. 4

Mechanical allodynia and thermal hyperalgesia in CD8+-depleted arthritic mice. Prior to arthritis induction, CD8+ cells were depleted by repeated i.p. injections of anti-CD8 antibodies (clone 2.43) until the end of the observation period. Control mice received the isotype-matched control antibody. a Success of CD8+ depletion was confirmed in the peripheral blood by flow cytometry. Paw withdrawal thresholds and paw withdrawal latencies of CD8+-depleted or isotype-treated arthritic mice were determined during the onset of chronic ACIA at the time points indicated in the ipsi- and contralateral paws using von Frey filaments (b) and Hargreaves test (c), respectively. Data are represented as means ± SEM. Statistical analysis was performed using the two-way RM ANOVA and Bonferroni’s multiple comparison test followed by a Bonferroni correction comparing ipsi- vs. contralateral sides of animals receiving anti-CD8 (***P < 0.001) or a control antibody (### P < 0.001); no significant differences were observed between ipsilateral thresholds of anti-CD8 and isotype control antibody-treated animals or between the contralateral paws of the two treatment groups, N = 8

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