Fig. 3

Dexamethasone protects FC neurons (a, b) and oligodendrocytes (c, d) from Bb-induced apoptosis. Representative images of apoptosis after immunofluorescence staining and visualized by confocal microscopy by the in situ TUNEL assay (green) in a neurons stained with NeuN (red), and c oligodendrocytes stained with S-100 (red). b, d Graphical representations of the effect of dexamethasone on the levels of apoptosis in neurons and oligodendrocytes, respectively, as induced by live Bb. The two-way ANOVA and Tukey’s multiple comparison test were used to evaluate the statistical significance between means and SD of ten data sets (approximately 500 cells) for each condition, **p < 0.01, ***p < 0.001