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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: The potential neuroprotective role of a histone deacetylase inhibitor, sodium butyrate, after neonatal hypoxia-ischemia

Fig. 5

Sodium butyrate decreases IL-1β expression in astrocytes. Seven-day-old rats (PND7) were subjected to hypoxia-ischemia followed by 6 days of recovery. SB or vehicle was administered directly after the onset of HI and for 5 consecutive days. Brain sections from ipsilateral hemispheres were stained for GFAP immunoreactivity (red) and for IL-1β (green). Nuclei were labeled with the Hoechst dye (blue). The majority of astroglial cells expressed IL-1β within the cortex of ipsilateral side. The amount of these cells was markedly reduced after SB administration. a Photomicrographs are representative of observations made from 5 animals per experimental group. Scale bar 100 μm. b Graph shows the percent of the GFAP(+)/IL-1β (+) cells versus total pool of GFAP-positive cells quantified in the frontal cortex (1.44 mm2 area). The values represent means ± SD of five animals per each experimental group. Student’s t test indicates significant differences in the number of GFAP(+)/IL-1β (+) cells between the investigated groups: ***p < 0.001. IPSI ipsilateral

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