Fig. 7

Effects of SB treatment on gene expression of IL-1β and TNFα in the brain after neonatal HI. Seven-day-old rats (PND7) were subjected to hypoxia-ischemia followed by 12, 48, and 72 h of recovery. SB or vehicle was administered directly after the onset of HI and for 2 or 3 consecutive days (determined by the experimental paradigm). The mRNA expression was determined in the ipsilateral (injured) hemispheres as well as in the control brains. Graphs represent statistical analysis of the data from indicated experimental groups: vehicle control (C), SB-treated control (C + SB), vehicle-treated hypoxia-ischemia (HI), and SB-treated hypoxia-ischemia (HI + SB). Note the decrease of HI-induced elevation of TNFα mRNA expression after SB treatment at 48 h of recovery. The values represent means ± SD from five animals in each group. The one-way ANOVA and Bonferroni test: *p < 0.05, **p < 0.01, and ***p < 0.001. C control, ipsi ipsilateral, contra contralateral