Fig. 6

BCG vaccination induced pia mater macrophage secretion of anti-inflammatory cytokine IL-10; the effect could be prevented by minocycline treatment. a Representative micrographs of the median sulcus of cortex stained for CD11b (green) and IL-10 (red) from the BCG Enr group. b A higher magnification (×63 and zoom 2.5) of the inset boxed area in a. c, e, f Confocal micrographs of ventral (c, f) and dorsal parenchyma (e) of the cortex stained for CD11b (green) and IL-10 (red) from the BCG Enr group. d A higher magnification of the inset boxed area in c. g Confocal micrographs of pia mater stained for CD11b and IL-10 from BCG Enr group. h Quantification of IL-10 mRNA levels of different groups. *p < 0.05, **p < 0.01, ***p < 0.001; Student’s t test for comparison of BCG Enr group and BCG Enr-treated minocycline group, and two-way ANOVA, LSD post hoc test for others; n = 6 per group. Scale bars 20 μm in a; 100 μm in c; 50 μm in d; 10 μm in b, e, f, and g. The data are presented as the means ± SEMs