Fig. 2
From: Regulatory role of cytosolic phospholipase A2 alpha in the induction of CD40 in microglia
cPLA2α is not involved in the induction of the BV-2 cells towards M2 phenotype induced by IL-4 + IL-10. A representative double-immunofluorescence staining of cPLA2α (green) and CD206 (red) in the unstimulated or stimulated BV-2 cells with IL-4 (20 ng/ml) + IL-10 (20 ng/ml) for 24 h in the absence or presence of AS or SE. DAPI staining shows cell nuclei. Scale bars = 100 μm. a The intensity of CD206 or cPLA2α were quantitated and expressed in the bar graph as arbitrary units. The bar graphs are the mean ± SE from three independent experiments. b A representative immunoblot analysis of arginase 1 protein expression in the cells treated as in a. The intensity of each Arg1 band after quantification by densitometry was divided by the intensity of each calreticulin band and expressed as arbitrary units. The bar graphs are the mean ± SE from three independent experiments. (***p < 0.0001, n.s. not significant)