Fig. 2

Protein analysis of the presence and levels of neurofilament light chains (NF-L) and inflammatory mediators in CSF. a NF-L protein levels have been analysed in the CSF of controls, OCB− and OCB+ MS patients by using ELISA assessment, showing significant increased levels in OCB+ MS patients compared to both controls and OCB− MS patients. b Cluster analysis showing the level of expression of the 28 inflammatory mediators examined in OCB− and OCB+ MS patients: the red rectangle outlines a cluster of molecules significantly overexpressed only in OCB+ MS patients. c Statistical representation of the presence and levels of inflammatory mediators significantly overexpressed in OCB+ compared to controls and to OCB− MS patients. (Statistical analysis performed by using Mann-Whitney test; p value *<0.05; **<0.01; ***<0.001)