Table 2 Methodological details of the examined inflammatory proteins in the CSF of MS patients by Bio-Plex immunoassay System
|  |  | Detection limit (pg/ml) | Intra-assay variation (%) | Recovery percentage range (90–110%) |
|---|---|---|---|---|
BCA1/CXCL13 | Chemokine (C-X-C motif) ligand 13 (CXCL13) or B lymphocyte chemoattractant (BLC) or B cell-attracting chemokine 1 (BCA-1) | 0.42–6694 | 6.0 | 100 |
SDF1αβ/CXCL12 | C-X-C motif chemokine 12 (CXCL12) or stromal cell-derived factor 1 (SDF1) | 12.73–160,858 | 5.4 | 104 |
6Ckine/CCL21 | Chemokine (C-C motif) ligand 21 | 11.30–160,686 | 4.0 | 104 |
IP10/CXCL10 | C-X-C motif chemokine 10 (CXCL10) or interferon gamma-induced protein 10 (IP-10) | 1.04–14,668 | 1.5 | 95 |
BAFF/TNFSF13B | B cell activating factor (BAFF) or tumour necrosis factor ligand superfamily member 13B (TNFSF13B) | 70.19–197,936 | 6.3 | 100 |
APRIL/TNFSF13 | A proliferation-inducing ligand (APRIL), or tumour necrosis factor ligand superfamily member 13 (TNFSF13) | 906.31–4,745,900 | 7.5 | 103 |
TWEAK/TNFSF12 | TNF-related weak inducer of apoptosis (TWEAK) or tumour necrosis factor ligand superfamily member 12 | 3.59–6346 | 4.4 | 106 |
LIGTH/TNFSF14 | Tumour necrosis factor superfamily member 14 (TNFSF14) | 1.92–4647 | 5.8 | 98 |
TNFα | Tumour necrosis factor alpha | 0.24–13,148 | 2.5 | 99 |
sTNFR1 | Soluble- tumour necrosis factor-receptor 1 | 2.33–48,047 | 5.0 | 100 |
sTNFR2 | Soluble- tumour necrosis factor-receptor 2 | 3.72–63,484 | 4.2 | 98 |
MIP3β/CCL19 | Chemokine (C-C motif) ligand 19 (CCL19) or EBI1 ligand chemokine (ELC) or macrophage inflammatory protein-3-beta (MIP-3-beta) | 3.08–49,150 | 2.8 | 103 |
MIG/CXCL9 | Chemokine (C-X-C motif) ligand 9 (CXCL9) or monokine induced by gamma interferon (MIG) | 1.19–23,643 | 5.4 | 97 |
sCD163 | Soluble-CD163 (Cluster of Differentiation 163) | 0.38–10,497 | 6.0 | 102 |
GM-CSF | Granulocyte-macrophage colony-stimulating factor (GM-CSF) or colony-stimulating factor 2 (CSF2) | 2.32–34,028 | 5.2 | 104 |
INFα2 | Interferon alpha-2 | 1.24–19,334 | 5.3 | 99 |
INFβ | Interferon beta | 1.50–8727 | 6.0 | 100 |
INFγ | Interferon gamma | 1.50–2326 | 3.0 | 99 |
IL1β | Interleukin-1 beta | 0.15–2326 | 2.0 | 102 |
IL2 | Interleukin-2 | 0.47–10,334 | 4.0 | 98 |
IL4 | Interleukin-4 | 0.35–5258 | 6.0 | 100 |
IL6 | Interleukin-6 | 0.68–57,162 | 3.0 | 99 |
IL8/CXCL8 | Interleukin-8 (C-X-C motif) or chemokine ligand 8, CXCL8) | 11.56–8523 | 1.0 | 103 |
IL10 | Interleukin-10 | 0.84–2997 | 3.0 | 98 |
OPN | Osteopontin | 123.64–286,066 | 7.3 | 100 |
MMP1 | Matrix metallopeptidase 1 | 298.2–216,430 | 4.5 | 100 |
MMP2 | Matrix metallopeptidase 2 | 90.63–2,109,030 | 3.3 | 104 |
MIF | Macrophage migration inhibitor factor | 8.25–456,461 | 2.6 | 103 |