Fig. 6
From: Cell-specific deletion of C1qa identifies microglia as the dominant source of C1q in mouse brain
No overt difference in expression of C1q in liver cells and kidney macrophages in C1qa FL/FL:Cx3cr1 CreERT2 compared to wild type mice, correlating with lack of YFP/Cre expression in these tissues in C1qa FL/FL:Cx3cr1 CreERT2. C1q staining of the liver (a) or kidney (b) WT (top panels), C1qa FL/FL :Cx3cr1 CreERT2 (middle panels). C1q reactivity is absent from C1qGT/GT (bottom panels) as expected demonstrating specificity of the antibody in these tissues. Representative pictures of n = 2–5 mice per genotype per age at 4–6 months. C1q (red) but not YFP (green) is detected in the liver (c) and kidney (d) of Cx3cr1 CreERT2 mice (4 months). Bottom panels of (c and d) are the merged images of C1q and YFP. Scale bars: 50 um