Fig. 7

C1q concentration is similar in the blood of C1qa ^FL/FL:Cx3cr1 ^CreERT2 and C1qa ^FL/FL littermates lacking Cx3cr1 ^CreERT2. a Compiled densitometry of western blot analysis of plasma (1 ul loaded per lane under reducing conditions) from a total of 82 animals: C1qa ^FL/FL:Cx3cr1 ^CreERT2 (dotted line, N = 5, 5, 8, and 19), C1qa ^FL/FL littermates (solid line, N = 3, 4, 8, and 10), and C57BL6/J (dashed line, N = 2, 5, 10, and 3). Plotted values for 4 and 8 months are pooled data from 3–5 and 7–10 months of age, respectively. Membranes were probed with rabbit anti-mouse C1q antibody (1151). Values are +/− SEM. Concentration was determined relative to known wild type plasma C1q concentration on each gel. No significant difference was observed between the groups by one-way ANOVA followed by Bonferroni’s multiple comparisons test (GraphPad). b The same analysis was performed for 5 months (gray) and 10 months (black) C57BL6/J, C1qa ^FL/FL, Arctic C1qa ^FL/FL, C1qa ^FL/FL:Cx3cr1 ^CreERT2, and Arctic:C1qa ^FL/FL:Cx3cr1 ^CreERT2, all in the absence of tamoxifen treatment. There are no statistical differences in plasma C1q concentration among these ages and genotypes, using one-way ANOVA with Bonferroni’s multiple comparisons test