Cell-specific deletion of C1qa identifies microglia as the dominant source of C1q in mouse brain

Table 1 Effect of Promoter Specific Cre^ERT2 Expression

Model	Target Gene	Cre construct	Tamoxifen^a	Effect on target gene expression^b
				IHC		Western blot		QPCR	Titer
				Brain	Liver Kidney	Brain	Blood	Brain	Blood
C57Bl6J	C1qa	Thy1 ^CreERT2	-	-		-	-		-
C57Bl6J	C1qa	Thy1 ^CreERT2	+	-		-	-		-
C57Bl6J	C1qa	Cx3cr1 ^{CreERT2/WganJ}	-	↓	-	↓	-	↓	-
C57Bl6J	C1qa	Cx3cr1 ^{CreERT2/WganJ}	+	↓	-	↓	-	↓	-
C57Bl6J	C1qa	Rosa26 ^CreERT2	-	-			-
C57Bl6J	C1qa	Rosa26 ^CreERT2	+	↓			↓
Arctic	C1qa	Cx3cr1 ^{CreERT2/WganJ}	-	↓		↓	-	↓
C57Bl6J	CAG-tdTomato	Cx3cr1 ^{CreERT2/WganJ}	-	↑^c

^a"-" indicates no treatment, "+" indicates Tamoxifen treatment as described in Materials and Methods
^b"-" indicates no change from wild type control and arrow down indicates decreased C1q
^cevidence of nuclear recombinase activity