Fig. 3

Expression of nestin-positive cells in the SVZ, DG, and stroke area (SA) regions. Schematic presentation of the stroke area (a). Analysis of brain sections by immunofluorescence revealed an increase in expression of nestin-positive cells of the SA (b–g), DG (h–j), and SVZ (k–m) 7 days after MCAO. Statistical analysis showed a significant increase of nestin-positive cells after MCAO, particularly at 7 days of MCAO in the DG (n), SVZ (o), and SA (p–q) (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). The number of GFAP-positive cells was also increased in the stroke area region at 3 and 7 days. The white arrowheads show the double positive cells. Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple test (n = 3–5). Scale bar: 100 μm