Fig. 8

Lipopolysaccharide-stimulated MP increase microglial activation in the cortex of uninjured mice. Enriched MP were isolated from control and LPS-stimulated BV2 microglia and were stereotactically injected into the cortex of adult male C57BL/6 mice. Iba-1 immunocytochemistry was performed at 7 days postinjection. a Representative Iba-1 staining (red) in the cortex (CTX), hippocampus (HP), and thalamus (TH). Images taken at −2.06 mm from the bregma; scale bar = 50 μm. b High-magnification images in control MP- and LPS MP-injected mice in the CTX, HP, and TH. LPS MP-injected Iba-1-positive microglia had enlarged cell body and thicker projection indicative of increased activation status. Scale bar = 100 μm. c Quantification of Iba-1 staining in the cortex, hippocampus, and thalamus at 7 days postinjection. There was a significant increase in Iba-1 immunoreactivity in the LPS MP-injected group when compared to the control MP-treated group (***p < 0.001 vs control MP; one-way ANOVA with Student-Newman-Keuls correction for multiple comparisons; n = 4/group). Bars represent mean ± S.E.M.