Fig. 9

Lipopolysaccharide-stimulated MP alter P2Y12 microglial morphology of the cortex of uninjured mice. Enriched MP were isolated from control and LPS-stimulated BV2 microglia and were stereotactically injected into the cortex of adult male C57BL/6 mice. P2Y12 immunocytochemistry was performed at 7 days postinjection. a High-magnification images of P2Y12-positive microglia (green) in control MP- and LPS MP-injected mice in the cortex (CTX), hippocampus (HP), and thalamus (TH). LPS MP-injected P2Y12-positive microglia have enlarged cell body and thicker projection indicative of increased activation status. Scale bar = 100 μm. b P2Y12-positive microglia in the CTX, HP, and TH of control MP- and LPS MP-injected mice. c Morphological analysis of P2Y12-positive microglia using 3D-reconstruction Neurolucida software. When compared to the control MP-injected group, P2Y12-positive microglia in the LPS MP-injected group had reduced ramification length in the cortex and hippocampus (**p < 0.01; Student’s t test), but not in the thalamus. In addition, the LPS MP-injected group had enlarged cell body area in each region (**p < 0.01 and ***p < 0.001 vs control MP; Student’s t test; n = 4/group). Bars represent mean ± S.E.M.