Fig. 6

Hypoxia-ischemia causes predominantly neuronal cell death. Brain sections were stained with DAPI to fluorescently label nuclear DNA content (a, e, i, m) of different cell types: neurons (NeuN, b), oligodendrocytes (Olig2, f), microglia (IBA-1, j) and astroglial (GFAP, n), as well as TUNEL assay (c, g, k, o). Overlay (OVL) of images showed clear colocalization of TUNEL and neuronal cells (d, white arrows), with minimal colocalization with oligodendrocytes (h) and microglia (l). There was no evidence of TUNEL colocalization with astroglial (p) cells. Please note the border between the basal ganglia (right) and periventricular white matter (left) in 4. d Demonstrating clear presence of TUNEL positive cells in the grey matter