Fig. 6

a ZIKV induction of proinflammatory and angiogenic cytokines in retinal microvascular endothelial cells after 96 h. Cytokine profiles of ZIKV-infected retinal endothelial cells by Luminex analysis at 96 h post infection are given. Results from cells exposed to media only are shown as solid black bars; cells exposed to heat-killed ZIKV are shown as gray bars; and results from cells exposed to the ZIKV are shown as stippled black bars. Results are included for β2-m (a-1), GMCSF (a-2), ICAM-1 (a-3), IL-4 (a-4), and IL-6 (a-5), MCP-1 (a-6), RANTES (a-7), and VCAM-1 (a-8). Results are given in picograms per milliliter. The results shown are the averages of replicate samples. b RANTES expression levels by qRT-PCR in retinal endothelial cells, retinal pericytes, and Müller cells infected with ZIKV over a time course of 24 and 96 h and 8 days after infection. Results are included for RANTES levels in retinal endothelial cells (b-1), RANTES levels in retinal pericytes (b-2), and RANTES levels in Müller cells (b-3). Colored bars indicate the normalized fold transcriptional expression of RANTES compared with mock-infected control cells. ND indicates no transcriptional expression detected