Astrocyte-specific overexpressed gene signatures in response to methamphetamine exposure in vitro

Table 2 List of the 30 most upregulated genes by each one of the three doses of Meth utilized to stimulate astrocytes

Genes upregulated by Meth in primary astrocyte cultures
	Dose 1 μM Meth	Fold change	Dose 10 μM Meth	Fold change	Dose 100 μM Meth	Fold change
1	SMG1	154.71	ZNF492	53.53	SLC12A1	124.70
2	LOC153077	49.55	KBTBD10	46.06	SMG1	120.32
3	MAP2K5	47.30	MAP2K5	40.66	CXCL11	72.41
4	POGZ	42.73	ADAM3A	39.21	TACR3	54.41
5	SSA2	37.28	GPR65	38.31	RBPMS	51.89
6	PHF3	30.59	KIAA0506	34.32	MAP2K5	51.74
7	ED1	28.51	EIF3S5	32.72	PTGER3	49.18
8	DKFZp547G183	27.90	FLJ21463	28.81	R29124_1	49.00
9	C13orf10	26.12	CXCL5	28.57	KIAA1579	48.75
10	ZNF198	25.51	PTGER3	27.56	PHF3	47.05
11	TNP2	23.46	FLJ11292	26.79	NAV3	43.44
12	NFAT5	23.00	SLC12A1	26.12	RGS13	42.76
13	CCL27	21.35	SAMSN1	25.74	SSA2	39.64
14	GAD2	19.96	NAV3	25.26	RIT2	39.33
15	XCL1	19.84	NSBP1	25.17	C14orf105	38.76
16	RW1	19.68	FLJ22595	25.09	RW1	35.53
17	NAV3	18.93	CCL27	24.51	IBTK	34.45
18	CXCL5	18.68	MLL4	20.74	CEACAM8	34.23
19	FLJ21463	18.35	FLJ13265	20.68	ED1	32.47
20	MDM2	17.42	MR1	20.62	FLJ21463	30.81
21	ERO1LB	17.38	KRTAP2-2	20.52	BLZF1	30.54
22	DEFA1	17.12	TAP2	20.47	ARTN	29.96
23	GLRA2	16.63	TRGC2	20.42	POGZ	29.02
24	DKFZp761K1824	16.25	ZNF198	20.32	CSHL1	28.22
25	FLJ11292	15.84	PC	19.67	CXCL5	27.58
26	MYO15B	15.81	ELL2	19.67	DLEU2	27.26
27	GPR65	15.67	TRIM29	19.48	SOX30	27.15
28	FLJ20045	14.96	TACR3	18.76	TAP2	27.07
29	ZNF407	14.94	ED1	18.13	GPR65	26.87
30	ADAM3A	13.97	CRISP1	17.94	TRGC2	26.81

The doses of Meth corresponded to low (1 μM), moderate (10 μM), and high (100 μM) Meth exposure. All genes in the list have been curated for significance as described in the Methods section. The genes in italics correspond to molecules that consistently appeared among the 30 upregulated genes in all three doses of Meth and, for this reason, were prioritized for validation.

ISSN: 1742-2094